Chronic endurance exercise affects paracrine action of CD 31 + and CD 34 + cells on 1 endothelial tube formation
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چکیده
26 We aimed to determine if chronic endurance exercise habits affected redox status and 27 paracrine function of CD34+ and CD34-/CD31+ circulating angiogenic cells (CACs). Subjects 28 were healthy, nonsmoking, men and women aged 18-35 yrs and categorized by chronic physical 29 activity habits. Blood was drawn from each subject for isolation and culture of CD34+ and 30 CD34-/CD31+ CACs. No differences in redox status were found in any group across either cell 31 type. Conditioned media (CM) was generated from the cultured CACs and used in an in vitro 32 HUVEC-based tube assay. CM from CD34+ cells from inactive individuals resulted in tube 33 structures that were 29% shorter in length (P<0.05) and 45% less complex (P<0.05) than the 34 endurance-trained group. CD34-/CD31+ CM from inactive subjects resulted in tube structures 35 that were 26% shorter length (P<0.05) and 42% less complex (P<0.05) than endurance-trained 36 individuals. Proteomics analyses identified S100A8 and S100A9 in the CM. S100A9 levels were 37 103% higher (P<0.05) and S100A8 was 97% higher in the CD34-/CD31+ CM of inactive 38 subjects compared to their endurance-trained counterparts with no significant differences in either 39 protein in the CM of CD34+ CACs as a function of training status. Recombinant S100A8/A9 40 treatment at concentrations detected in inactive subjects’ CD34-/CD31+ CAC CM also reduced 41 tube formation (P<0.05). These findings are the first, to our knowledge, to demonstrate a 42 differential paracrine role in CD34+ and CD34-/CD31+ CACs on tube formation as a function of 43 chronic physical activity habits and identifies a differential secretion of S100A9 by CD3444 /CD31+ CACs due to habitual exercise. 45 46 47 48 49 50
منابع مشابه
Chronic endurance exercise affects paracrine action of CD31+ and CD34+ cells on endothelial tube formation.
We aimed to determine if chronic endurance-exercise habits affected redox status and paracrine function of CD34(+) and CD34(-)/CD31(+) circulating angiogenic cells (CACs). Subjects were healthy, nonsmoking men and women aged 18-35 yr and categorized by chronic physical activity habits. Blood was drawn from each subject for isolation and culture of CD34(+) and CD34(-)/CD31(+) CACs. No difference...
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